APLICATION OF ANTI PREGNANCY-ASSOCIATED GLYCOPROTEIN (ANTI-PAG) AS A MOLECULAR MARKER OF EARLY PREGNANCY DETECTION IN DAIRY HEIFER

Tita Damayanti Lestari

Abstract
Research on Aplication of Anti Pregnancy-Associated Glycoprotein (Anti-PAG) as a molecular marker of early pregnancy detection in dairy heifer was conducted to support the dairy farmer in order to achieve efficiency from their heifer reproduction. The goal of research was to proove that anti-PAG could recognize antigen PAG in early pregnant dairy serum. Specificity test was done qualitatively by Dot Blot method. The collected data were visualized with purple color which derived from specific reaction between antigen (PAG) and antibody (anti-PAG). Expression of color was converted to numbers by NIH Image software, Version 1.62. Numeric average represent result of Dot Blot reaction. Collected data were statistically analysed by Nested Completely Random Design software. Result showed Anti-PAG recognition on antigen PAG in pregnant dairy heifer blood serum, from the 15^th day of pregnancy. Consequently, Anti-PAG can be used as a molecular marker of an early pregnancy detection in dairy heifer.

Key words: Anti-PAG, Dot Blot, early pregnancy, detection, dairy heifer