PROFILE OF BLASTOCIST PROTEIN: PREGNANCY-ASSOCIATED GLYCOPROTEIN (PAG) AS AN INDICATOR OF PREGNANCY IN LIVESTOCK

Tita Damayanti Lestari, Ismudiono Ismudiono

Abstract
The research on Pregnancy-Associated Glycopotein (PAG), a blastocyst protein isolated from pregnant cow serum was conducted to find out its characterisation. The aim of the research was to study the PAG profile as an indicator of pregnancy in dairy cattle. PAG protein was isolated from serum from cows during 274-279 days of gestation, prior to deliver through an isolation and characterisation technique such as SDS-PAGE, Western Blot, Electroelusion and ELISA. The results of research show that PAG isolated from pregnant dairy cows in East Java had molecular weight of 67.34 kDa.

Key words: Pregnant Cow Serum, Blastocyst Protein, PAG and Characterization