ESTABLISHING SOME MORPHOMETRIC PARAMETERS USABLE IN ESTIMATING THE QUALITY OF MOUSE EMBRYOS, IN DIFFERENT DEVELOPMENTAL STAGES
N. Păcală, Ada Cean, Alexandra Boleman, Rodica Căpriţă, I. Bencsik, D. Dronca, Gabi Dumitrescu
Abstract
The aim of this paper was to establish some morphometric parameters that can be used in estimation of mouse embryos quality in different developmental stages. For experiments we used embryos recovered from mouse females superovulated with gonadotrope hormones (PMSG and hCG). Embryos recovery was performed at different time intervals after the vaginal plug was discovered. The morphometric parameters taken into consideration were: pellucid zone thickness, outer and inner diameter, and outer and inner perimeter. The embryos recovered were measured for establishing morphometric parameters and in vitro cultivated in KSOM media, supplemented with amino acids. The pellucid zone thickness at the embryos in morula stage (10.8±1.9µm) was higher compared with the embryos in 2 cell stage (9.8±1.9 µm) and blastocyst stage (7.3±1.5 µm); the differences were very significant (p≤0,001, T test). The inner diameter at the embryos in blastocyst stage (111.3±5.8 µm) was larger comparative with the embryos in 2 cell stage (98.5±2.5 µm) and embryos in morula stage (99.4±3.1 µm); the differences were very significant (p≤0,001, T test). The outer diameter at the embryos in blastocyst stage (123.1±3.5 µm) was larger comparative with the embryos in 2 cell stage (116.5±3.3 µm) and embryos in morula stage (111.7±3.7 µm); the differences were very significant (p≤0,001, T test).
