EFFECT OF GRAPE POMACE EXTRACT ENRICHED IN POLYPHENOLS IN COUNTERACTING INTESTINAL INFLAMMATION BY USING AN IN VITRO INTESTINAL CELLULAR MODEL

Gina Cecilia Pistol, Daniela Eliza Marin, Ionelia Taranu

Abstract
    The rapidly increase of the incidence of severe chronic intestinal inflammation diseases have a huge impact on animal and human life quality. The counteracting of intestinal inflammation using prebiotics was lately investigated. Grape pomace (GP) is an industrial waste from the wine processing rich in polyphenols which could be an alternative, less expensive source of anti-inflammatory bioactive compounds (polyphenols). Using an in vitro model of intestinal inflammation, we evaluated the effect of the grape pomace extract enriched in polyphenols on several key markers of inflammation. Thus, expression of cytokines, TLRs and signalling molecules were analysed in epithelial CaCO2 cell culture. After cell culture treatments, tRNA was extracted and gene expressions were evaluated by qPCR. Our results showed that grape pomace extract is able to counteract the LPS-induced intestinal inflammation by decreasing the gene expression of pro-inflammatory cytokines: TNF-Α, IL-1Β, IL-6 and Il-8 and by slightly increase of the IL-10, anti-inflammatory cytokine mRNA. Also, our results showed that grape pomace extract could modulate the expressions of TLRs genes, as modulators of the innate immune response.
    In conclusion, our study contributes to a better understanding of the grape pomace extract enriched in bioactive compounds, in modulation the intestinal inflammation, as well as the consequence in terms of susceptibility to enteric disease.

Key words: intestinal inflammation, grape pomace, polyphenols, cellular model