EVALUATION OF THE POST-CLOSURE CONTAMINATION DEGREE IN THE BROILER OF HERBAL

Elena Hriscu (Ursu), Irina Elena Ismană (Ciobotaru), M.G. Usturoi

Abstract
    The study was conducted on two growing farms, from which 80 samples (one day old chicks) were taken, half in the hot season and half in the cold season; practically, 40 samples were collected per season (2 popular series x 40 samples / season, ie 80 samples in the two seasons of two different farms).
    Parameters analyzed were: Salmonella spp., Escherichia coli, Staphylococcus spp., Streptococcus spp. Using the national, standardized, validated and accredited RENAR working methods. Detection and isolation of bacterial strains was done on liquid and solid culture media for pre-enrichment, enrichment, isolation and biochemical identification. For confirmation, mini-API galleries specific to each category of bacteria were also used: Salmonella spp. and Escherichia coli ID 32 E; for Staphylococcus spp - galleries ID 32 STAPH and for Streptococcus spp - rapid ID 32 STREP.
    From 40 samples analyzed in the summer season, the two growing farms resulted in 85% contaminated samples.
    From the winter season, we examined 40 samples from the same growing farms and resulted in 10% of the contaminated samples.
    From the analyzes performed, it emerged that day-old chicks were contaminated with the same types of bacterial strains, Escherichia coli and Staphylococcus spp., From both growing farms.
    The highest incidence was with the Escherichia coli strain, at a rate of 70%; And the strains of Staphylococcus spp were found to be 25%.

Key words: isolation, identification, bacterial strains, day-old chicks