COMPARATIVE STUDY ON THE USE OF VARIOUS HORMONAL PREPARATIONS (CARP PITUITARY, NERESTIN 6A) IN THE ARTIFICIAL REPRODUCTION OF PERCA FLUVIATILIS

C.-A. Barbacariu, Lenuţa Dirvariu, M. Burducea, C. Mihai

Abstract
    The objective of this experiment was to test the use of hormonal preparations, which are commonly used in the artificial reproduction of various fish species, on Perca fluviatilis in order to develop and optimize an artificial reproductive technology under controlled conditions. The hormonal preparations used in the experiment were carp hypophysis and Nerestin 6A, and the biological material consisted of 20 females and 6 males.
    The females were divided into two lots: (L1) carp hypophysis (4 mg / kg female body weight) and (L2) Nerestin 5A (0.25 ml / kg females body weight). Hormonal stimulation was done by intraperitoneal syringe administration for the carp hypophysis and intramuscular injection for Nerestin 5A, both in two divided doses as follows: 20% of the total dose in the first injection and 80% in the second.
    Males did not require hormonal stimulation. The average body weight of females was 399.1 ± 9.66 g (L1) and 425.10 ± 25.77 (L2).
    The average latency time between the first injection and the ovulation time was 121.43 ± 10.87 hours in L1 and 84.71 ± 6.12 hours in the case of L2. The mean value of the relative fecundity index was 209±66.5 x10³ eggs/kg in L1 and 273.1±34.5 x10³ eggs/kg in L2. The mean value of the absolute fecundity index was 87.2±23.4 x10³ eggs/ female in L1 and 109±11.1 x10³ eggs /female in L2. The fertility rate had average values of 87.80 ± 2.63 for group L1 and 91.14 ± 2.87 for L2. The survival of females during the experiment was 80% in the L1 group, 90% in the L2 group and 100% in the males. The hatching rate averaged 36% in the first group (L1) and 52% in the second group (L2). Significant statistical differences between the two groups were recorded for the amount of harvested eggs and latency time, these indicators being higher for the L2 group, while insignificant differences were recorded for the other indicators.
    The results have shown that artificial reproduction is possible under controlled conditions and can be used to develop aquaculture of the species.

Key words: artificial reproduction, perch, nerestin 5A, carp pituitary