RESEARCHES REGARDING THE CRYOPRESERVATION OF THE SPERM OF THE BOAR

Elena Cibotaru, G. Darie, Doina Rotari

Abstract
   The research was conducted on the boar sperm. The semen was collected by manual method. After collecting the sperm was subjected to the macroscopic and microscopic examination. Ejaculates admitted for processing were diluted with dilution media in which the CL-1 substance with 7% antioxidant and membranotropic properties was added as an additional component. After dilution, the semen was refrigerated for 4 hours at + 2-4°C. After the term of refrigeration has expired, the semen has frozen in the form of granules. Defrosting was performed in a marine bath at 45°C. Testing of the defrosted material was performed by the CEROS computerized method. The mobility of sperm after thawing was 34-38% with viability at 37°C for 3-5 hours.

Key words: Boar, sperm, ejaculate, substance, dilution, mobility, viability