RESEARCH ON THE INFLUENCE OF "BIOR" PREPARATION ON CRIOCONSERVATION OF RAM SPERM

G. Darie, Nina Bradu, I. Iurcu

Abstract
   The object of the study was the sperm of sheep-producers of local breeds and the drug BioR (developed by the Institute of Microbiology and Biotechnology of the Academy of Sciences of Moldova). The drug BioR was added to the commercial environment (GCJ) as an additional component in a concentration of from 1 to 10%. It has been proven that BioR is not toxic to sperm within the studied concentration. The best results in motility and survivability after thawing of sperm were obtained when BioR at a concentration of 6% was introduced into the commercial medium of GC as an additional component. The medium was tested under production conditions for insemination of sheep. It was found that when using a medium in which BioR was introduced as an additional component, the fertility of sheep inseminated by thawed seed was 47.8%. A synthetic sperm cryopreservation medium for rams consists of glucose, sodium citrate, glycerin, egg yolk, BioR and antibiotics (patent 4513).

Key words: cryopreservation, environment, semen, insemination, ram