IN VITRO EVALUATION OF PROBIOTIC PROPERTIES OF A LACTIC ACID BACTERIA ISOLATED FROM PIGLET

Nicoleta-Raluca Chelaru, Mihaela Dumitru, I. Sorescu, Mihaela Hăbeanu

Abstract
   The aim of this study was to evaluate the probiotic aspects of lactic acid bacteria (LAB) isolated from faeces of a healthy piglet (30-d-old, Topigs). Cultural, morphological and biochemical characteristics by standard API 50 CHL Biomerieux strips of isolated strain was assayed as well. The strain was conserved as Lactobacillus fermentum 1 IBNA 95 and can be found in the collection of INCDBNA. It is a Gram positive, non-spore forming, aerotolerant bacteria. It appears isolated and grew at 37°C in Oxoid MRS broth and MRS agar medium. The functional properties tested were to survive at pH 3.0 and to present resistance at addition of 0.3% bile salts (w/v). The strain was able to survive at a low pH value and to tolerate 0.3% bile salt solution during 3h exposure under according simulated gastrointestinal condition. Lactobacillus fermentum 1 IBNA 95 was presenting some probiotic features, thus can be a promising feed supplement for piglet’s nutrition.