Abstract
Mastitis is a disease in cattle caused by E. coli bacteria. Pangalengan is a center for dairy farming that is still commonly found in cases of mastitis. The handling of mastitis cases is done by giving antibiotics. This study describes the detection of resistant antibiotics in E. coli that causes mastitis in milk and the livestock environment including water and cage floors. 192 subclinical mastitis milk samples, 9 water samples, and 9 cage floor swab samples were taken, identification of E. coli by planting on MacConkey agar and Levine-EMB agar, gram staining and biochemical testing of indole test. After that, antibiotic resistance testing is done using the Kirby Bauer method. The results showed that the highest resistance to Ampicillin (100%) in all samples, Sulphamethoxazole-trimethoprim (83%), and Oxytetracycline (66%) in the cage floor samples. This shows that the use of antibiotics is inappropriate and uncontrolled so that antibiotic resistance appears in E. coli in Pangalengan cattle farms, West Java.
Key words: Mastitis, Antimicrobial resistance, E. coli