BLOOD DNA EXTRACTION FOR GENETIC ANALYSIS IN ENDANGERED ROMANIAN GREY CATTLE

Mădălina A. Davidescu, A.C. Grădinaru, Daniela E. Ilie, Mihaela Ivancia, Şt. Creangă

Abstract
   The quality and quantity of DNA extracts used for genetic analysis are of utmost importance. The isolation of DNA from different types of samples can be done by classical methods or using specific kits, whose working methodology must strictly follow the protocol established by the manufacturer. The aim of this research was to evaluate the effectiveness of DNA extraction from 50 blood samples of Romanian Grey cows found in various genetic conservation programs due to their small number in cattle herds. The obtained results are considered satisfactory in terms of the purity of the obtained DNA extracts, with only one sample (2% of the total) having the DNA isolate contaminated with proteins based on the absorbance ratio A260/A280 lower than 1.8.