CHANGES INDUCED BY SOME NUTRITIONAL FACTORS ON FEMUR HISTOLOGICAL STRUCTURE IN PIG
Gabi Dumitrescu, Lavinia Stef, D. Drinceanu, Liliana Petculescu Ciochina, D. Stef, D. Dronca, Rodica Criste, Liliana Boca, C. Julean
Abstract
The problems approached by our team are represented by the involvement of different sources and levels of calcium, namely calcium carbonate, fructoborate and alfalfa, in the mineralization of bone tissue. So, we made fix histological preparations of tissue fragments taken from femure, from 9 pigs belonging to three batches: the control batch, where calcium was provided in a proportion of 1% through calcium carbonate, experimental batch 1, where calcium was provided in a proportion of 1.04% through fructoborate, on a calcium carbonate support, and experimental batch 2, where calcium was provided in a proportion of 1.13% through fructoborate + alfalfa, on a calcium carbonate support. The histomorphometric parameters assessed were represented by the volume of bone trabeculae (BV/TV, %) or the percentage of bone tissue in a given volume and the mean width of bone trabeculae. At the same time, in order to establish fructoborate and alfalfa implication in bone mineralization, we supervised the presence and activity of osteoblasts respectively osteoclasts. In the case of the experimental batch 1, the histomorphometric study shows an increase of bone trabeculae dimension, with a mean width of about 115.4μ μ, and also an increase of their mean volume, which is about 36.46%. The trabecular system is dense and present mineralised and ossified territory where are formed by osteoclasts with osteocytes. Peritrabeculary are presented active osteoblasts which are involved in plurilamellar stratification by deposition of young collagen. In the case of individuals from experimental batch 2, trabeculae mean width is about 111,5μ, while their volume is 33.60%.
MODIFICARI INDUSE DE UNII FACTORI NUTRITIONALI ASUPRA STRUCTURII HISTOLOGICE A FEMURULUI LA PORC
Gabi Dumitrescu, Lavinia Stef, D. Drinceanu, Liliana Petculescu Ciochina, D. Stef, D. Dronca, Rodica Criste, Liliana Boca, C. Julean
Rezumat
Problematica abordata de colectivul nostru vizeaza implicarea unor surse si niveluri diferite de calciu si anume carbonatul de calciu, fructoboratul si lucerna, asupra mineralizarii tesutului osos. În acest sens s-au realizat preparate histologice fixe din fragmente tisulare recoltate din femur, provenite de la 9 porci apartinând la trei loturi: martor la care calciul s-a asigurat în proportie de 1% prin carbonat de calciu, experimental 1, la care calciul s-a asigurat în proportie de 1,04% prin Fructoborat pe suport de carbonat de calciu si experimental 2, la care calciul s-a asigurat în proportie de 1,13% prin Fructoborat + lucerna pe suport de carbonat de calciu. Parametri histomorfometrici evaluati au fost volumul trabeculelor osoase (BV/TV, %) sau procentajul tesutului osos dintr-un volum dat si grosimea medie a trabeculelor osoase. Totodata, pentru a stabili implicarea frucoboratului si lucernei în mineralizarea osoasa, s-a urmarit prezenta si activitatea osteoblastelor, respectiv a osteoclastelor. În cazul lotului experimental 1, studiul histomorfometric arata o crestere atât a dimensiunii trabeculelor osoase, acestea având grosimea de cca. 115,4μ, cât si a volumului acestora la cca. 36,46%. Trabeculele au aspect compact si prezinta teritorii de depunere osoasa si de mineralizare, în urma carora se formeaza osteoplaste cu osteocite. Peritrabecular, se dispun osteoblastele active, implicate în stratificarea plurilamelara, prin depunere de colagen tânar. În cazul indivizilor din lotul experimental 2, grosimea trabeculelor osoase a fost de cca. 111,5μ, iar volumul acestora de 33,60%.
