BIOCHEMICAL DIAGNOSTICS OF OSTEODYSTROPHY OF GOATS

Y. Maslak, O. Mitrofanov, A. Sobakar

Abstract
    The purpose of this study was to research comparative analysis of data that have been obtained after clinical examination of goats and analysis of biochemical research of the level of metabolites of bone tissue metabolism in blood serum and urine for determination of criteria at diagnostics of osteodystrophy. The material of the study consisted of 20 adult goats. Diagnosis of was made according to physical examination of goats and analysis of biochemical research of the level of metabolites of bone tissue metabolism in blood serum and urine. Activity of aminotransferase (alanine aminotransferase, aspartate aminotransferase) and alkaline phosphatase and also concentration of glycoproteins (GP), chondroitin sulfate, fractional content of glucosaminoglycans, content of total and ionized calcium were assessed in blood serum. The content of hydroxyproline and total uronic acid were analyzed in urine. Comparative analysis of total clinical status of goats and level of biochemical components in blood serum and urine that characterize the state of connective tissue at diagnostics of osteodystrophy was conducted. The intergroup significant differences of some biochemical parameters (aspartate aminotransferase, alkaline phosphatase, chondroitin sulfate, fraction IІ mucopolysaccharides, sum of fractions glucosaminoglycans) were determined. Further investigation must be conducted to determine the biochemical parameters in goat with subclinical signs of osteodystrophy.

Key words: osteodystrophy, goats, blood serum, urine

DIAGNOSTICUL BIOCHIMIC AL OSTEODISTROFIEI LA CAPRINE

Y. Maslak, O. Mitrofanov, A. Sobakar

Rezumat
    Scopul acestui studiu a fost de a investiga comparativ datele culese prin examen clinic şi examen biochimic al metaboliţilor ţesutului osos, circulaţi în ser sanguin şi excretaţi prin urină, ca elemente de diagnostic al osteodistrofiei. Materialul biologic a cuprins un efectiv de 20capre adulte. Diagnosticul a fost stabilit prin examen fizic şi prin analize biochimice ale nivelurilor diverselor substanţe-indicatori ale metabolismului osos: transaminazele (alanin-aminotransferaza, aspartat-aminotransferase), fosfataza alcalină, concentraţia în glicoproteine (GP), condroitin-sulfatul şi conţinutul fracţiunilor glucozaminoglicanice, conţinutul de calciu total şi ionic din serul sanguin; hidroxiprolina şi acidul uronic total din urină. Aceste date au servit la efectuarea unei analize comparative între aspectele clinice şi biochimice care fac parte din tabloul patologic şi diagnosticul osteodistrofiei. S-au identificat diferenţe semnificative intergrup pentru unii parametri biochimici (aspartat aminotransferaza, fosfataza alcalină, condroitin sulfatul, fracţiunea II a mucoloplizaharidelor, suma fracţiunilor glucozaminoglicanilor). Crecetările vor continua, cu scopul identificării acelor parametri biochimici ce pot fi utilizaţi în diagnosticul osteodistrofiei subclinice.

Cuvinte cheie: osteodistrofie, caprine, ser sanguin, urină