RESEARCHES REGARDING THE CRYOPRESERVATION OF THE SPERM OF THE BOAR

Elena Cibotaru, G. Darie, Doina Rotari

Abstract
   The research was conducted on the boar sperm. The semen was collected by manual method. After collecting the sperm was subjected to the macroscopic and microscopic examination. Ejaculates admitted for processing were diluted with dilution media in which the CL-1 substance with 7% antioxidant and membranotropic properties was added as an additional component. After dilution, the semen was refrigerated for 4 hours at + 2-4°C. After the term of refrigeration has expired, the semen has frozen in the form of granules. Defrosting was performed in a marine bath at 45°C. Testing of the defrosted material was performed by the CEROS computerized method. The mobility of sperm after thawing was 34-38% with viability at 37°C for 3-5 hours.

Key words: Boar, sperm, ejaculate, substance, dilution, mobility, viability

CERCETĂRI PRIVIND CRIOCONSERVAREA SPERMEI DE VIER

Elena Cibotaru, G. Darie, Doina Rotari

Rezumat
   Cercetările au fost efectuate pe sperma de vier. Recoltarea materialului seminal s-a efectuat prin metoda manuală. După recoltare sperma a fost supusă examenului macroscopic şi microscopic. Ejaculatele admise pentru prelucrare au fost supuse diluării cu medii de diluţie în componenţa cărora a fost întrodus ca component suplimentar substanţa CL-1 cu proprietati de antioxidant şi membranotrop în concentraţie de 7%. După diluare materialul seminal a fost supus refrigerări timp de patru ore la temperature de +2-+4°C. După expirarea termenului de refrigerare materialul seminal s-a congelat în formă de granule. Decongelarea s-a efectuat în baie marină la temperature de 45°C. Testarea materialului seminal decongelat s-a efectuat prin metoda computerizată CEROS. Mobilitatea spermatozoizilor dupa decongelare a fost de 34-38% cu o viabilitate la temperature 37°C de 3-5 ore.

Cuvinte cheie: vier, material seminal, ejaculate, substanţă, diluant, mobilitate, viabilitate